Journal: Cell stem cell

Article Title: Human iPSC-derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-Tumor Activity

doi: 10.1016/j.stem.2018.06.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-IκBα , cell signaling technology , Cat#9242 RRID: AB_823540.

Techniques: Virus, Expressing, Plasmid Preparation, Recombinant, Transduction, Apoptosis Assay, Cell Isolation, Membrane, Protein Extraction, Enzyme-linked Immunosorbent Assay, Sequencing, Software, Imaging

Journal: Blood

Article Title: Monocytic microparticles activate endothelial cells in an IL-1?-dependent manner

doi: 10.1182/blood-2011-01-330878

Figure Lengend Snippet: MPs released by LPS-treated THP-1 cells stimulate intracellular signaling in HUVECs. A total of 100 μg/mL of MPs from LPS-treated (A) or untreated (B) THP-1 cells was incubated in serum-free conditions with HUVECs. Phosphorylation of ERK1/2 and degradation of IκB-α were observed by Western blot at the indicated times. (C) Indicated amounts of MPs from LPS-treated THP-1 cells or 10 ng/mL of TNF-α were incubated in serum-free conditions with HUVECs for the indicated time. Phosphorylation of p65 was detected by Western blot. (D) Subcellular localization of p65 was examined in HUVECs after 1 or 2 hours of stimulation with MPs from untreated or LPS-treated THP-1 cells or 10 ng/mL of TNF-α. HDAC2 served as the nuclear loading control, and GAPDH served as a cytoplasmic loading control. Results are representative of 2 independent experiments.

Article Snippet: Rabbit polyclonal antibodies against ERK1/2 (#9102), phospho-p65 (#3031), and IκB-α (#9242) were from Cell Signaling.

Techniques: Incubation, Phospho-proteomics, Western Blot, Control

Journal: The EMBO Journal

Article Title: The innate immune receptor MDA 5 limits rotavirus infection but promotes cell death and pancreatic inflammation

doi: 10.15252/embj.201696273

Figure Lengend Snippet: Micrographs showing the cellular distribution of IRF3 (green) in WT and Ifih1 −/− MEFs detected with an anti‐IRF3 antibody 4 h after infection with RV. Arrows indicate the more intense cytosolic, perinuclear enrichment of IRF3 in the WT and Ifih1 −/− cells before infection. This fluorescence pattern changes to a relatively more intense nuclear (blue) staining of IRF3 in RV‐infected WT cells but not infected Ifih1 −/− cells. The nucleus is visualized with the Hoechst DNA stain.

Article Snippet: Primary antibodies used for Western blots were as follows: mouse anti‐Mda5 (Axxora, ALX‐210‐352), anti‐IκBα (Cell Signaling, 9242), mouse anti‐Il‐1β (Abcam, ab9722), anti‐β‐actin (Abcam, ab8226), mouse anti‐P56 (Dr Ganes C Sen, Cleveland Clinic, USA), and anti‐GFP (SIGMA).

Techniques: Infection, Fluorescence, Staining